Isolation, selection and characteristics of strain for enzymatic degumming on Apocynum vernetum L.

Abstract

The plant Apocynum vernetum L. retted in Chaidamu basin of Qinghai province as the test sample. Domesticated and isolated are two highly productive pectinase strain suitable for degumming on Apocynum vernetum L. and following that, one strains is determined to be a new Acinetobacter junii and another is Bacillus subtilis, both of which are produced by pectinase through identification on the basis of physiological and biochemical index as well as the shape index and 16S rDNA strain identification. The hydrolysis circle experiment finds that the H/C value for the former (H/C is the rations of ring diameter to that of bacterial colony) is 5, and that for the latter is 3; the test of enzyme activity tells that Acinetobacter junii will be at the peak (103.2 lU/ml) at llh and Bacillus subtilis at 9h at the same temperature of 37 °C, moreover, the enzyme activity of the former is 12.5 % higher than that of the latter.